IL-17A 是一种同型二聚体细胞因子，在宿主防御机制中对许多细菌和真菌病原体以及过敏和自身免疫反应起着关键作用。IL-17A 诱导抗菌肽、细胞因子、趋化因子和基质金属蛋白酶的产生。Mouse IL17A Recombinant Protein (N-His) 是一种重组小鼠 IL-17A 蛋白，在 E. coli 中表达，由 137 个氨基酸组成(T22-A158)。
| 生物活性 |
1. Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα and the ED50 is 0.15-0.6 ng/mL. |
|---|---|
| 运输条件 |
Shipping with dry ice. |
| 储存方式 |
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles. |
| 研究背景 |
Interleukin-17A (IL17A) is a ~17 kDa protein. Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. IL17A is the therapeutic target of secukinumab (Cosentyx).
|
| 种属 |
Mus musculus (Mouse)
|
| 表达系统 |
E. coli
|
| Accession号 |
Q62386
|
| 蛋白长度 |
Thr22-Ala158
|
| 应用 |
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
|
| 预测分子量 |
17.67 kDa
|
| 性状 |
Lyophilized
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| 保存溶液 |
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
|
| 纯度 |
>90% as determined by SDS-PAGE.
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