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Mouse KLB/Beta-klotho Recombinant Protein (N-His) (Synonyms:Klotho beta-like protein;KLB;Beta-klotho; BetaKlotho;BKL)
目录号 : KM32454
Recombinant Mouse KLB/Beta-klotho Protein, N-His expressed in E. coli, spanning Ser86-Gly505. Purity: >90% by SDS-PAGE.
规格 价格 是否有货 数量
50μg
In-stock
100μg
In-stock
1mg
In-stock
KKL Med 的所有产品和服务仅用于科学研究,不能被用于人体,兽医,我们也不向个人提供产品和服务。
运输条件
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
储存方式
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
研究背景
Beta-klotho (KLB) is a ~120 kDa protein. Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.
种属
Mus musculus (Mouse)
表达系统
E. coli
Accession号
Q99N32
蛋白长度
Ser86-Gly505
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
预测分子量
50.82 kDa
性状
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
纯度
>90% as determined by SDS-PAGE.
暂无相关参考文献
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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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动物实验计算换算器
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系客服为您提供正确的澄清溶液配方)
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计算结果:

工作液浓度 mg/ml;

DMSO母液配制方法 mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,

体内配方配制方法μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。

配置后的溶液总体积

1. 首先保证母液是澄清的;
           2. 一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。