Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain.
IC50&Target
Dye reagent
DNA Stain
体外研究
Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI.
分子式
C27H28N6O.3[HCl]
分子量
561.93
CAS号
875756-97-1
运输条件
Room temperature in continental US; may vary elsewhere.