SP-96 - CAS:2682114-54-9

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SP-96

目录号 : KM8667 CAS No. : 2682114-54-9 纯度 : 98%

Data Sheet 产品使用指南

SP-96 是一种高效、选择性和 ATP 竞争性 Aurora B 抑制剂，IC₅₀ 为 0.316 nM，对 FLT3 和 KIT 具有 >2000 倍选择性。SP-96 在 NCI60 筛选中表现出选择性生长抑制，对 MDA-MD-468 等乳腺癌细胞具有选择性 (GI₅₀=107 nM)。SP-96 可用于三阴性乳腺癌 (TNBC) 的相关研究。

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500mg	询价	In-stock

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生物活性

SP-96 is a highly potent, selective and non-ATP-competitive Aurora B (IC₅₀=0.316 nM) inhibitor and shows >2000 fold selectivity against FLT3 and KIT. SP-96 shows selective growth inhibition in NCI60 screening, incluing MDA-MD-468 (GI₅₀=107 nM). SP-96 can be used for the research of triple negative breast cancer (TNBC).

体外研究

SP-96 is a highly potent, selective and non-ATP-competitive Aurora B (IC₅₀=0.316 nM) inhibitor and shows >2000 fold selectivity against FLT3 (IC₅₀=1475.6 nM) and KIT (IC₅₀=1307.6 nM).
SP-96 (0-1 µM; 24 hours) is not promiscuous, rather selective for a few cell lines, it inhibits MDA-MB-468, CCRF-CEM, COLO 205 and A498 cell growth with GI₅₀ values of 107 nM,47.4 nM, 50.3 nM and 53.2 nM, respectively.
SP-96 (63.2 nM) inhibits Aurora B activity in H460 cells by the characteristics of increased DNA content, and it increases cell volume with enormous nucleus.
SP-96 (0-2 µM) inhibits Aurora B enzymatic activity with an IC₅₀ of 0.316 nM and inhibits Aurora A with observed IC₅₀ value of 18.975 nM. SP-96 shows >2000 fold selectivity against FLT3 (IC₅₀=1475.6 nM) and KIT (IC₅₀=1307.6 nM). Meanwhile, it exhibits inhibitory effects on other receptor tyrosine kinases (RTKs) namely EGFR, RET and HER2 with IC₅₀ value ≥2 µM.

Cell Viability Assay

Cell Line:	MDA-MB-468, CCRF-CEM, COLO 205 and A498 cell
Concentration:	0-1 µM
Incubation Time:	24 hours
Result:	Showed good inhibitory activity on MDA-MB-468 cells.

分子量

453.47

CAS号

2682114-54-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 100 mg/mL (220.52 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.2052 mL	11.0261 mL	22.0522 mL
5 mM	0.4410 mL	2.2052 mL	4.4104 mL
10 mM	0.2205 mL	1.1026 mL	2.2052 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (5.51 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (5.51 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。