5β-Dihydrocortisol, a metabolite of Cortisol, is a potential mineralocorticoid. 5β-Dihydrocortisol can potentiate glucocorticoid activity in raising the intraocular pressure. 5β-Dihydrocortisol causes breast cancer cell apoptosis.
体外研究
5β-Dihydrocortisol (10-100 μM; 48 h) inhibits the viability of MCF-7 cells, with an IC50 of 27.59 μM.
5β-Dihydrocortisol (14 μM; 24 h) induces 35.6% early apoptosis and 2.5% late apoptosis in MCF-7 cells.
5β-Dihydrocortisol (1-10 μM; 48 h) quenches the intrinsic fluorescence of human serum albumin (HAS) with a maximum emission peak at 360 nm, with no shift in fluorescence peak.
Cell Viability Assay
Cell Line:
MCF-7 and HEK 293 cells
Concentration:
10, 20, 40, 60, 80, 100 μM
Incubation Time:
48 hours
Result:
Inhibited the viability of MCF-7 cells in a dose-dependent manner.
No toxicity in terms of cell viability was observed with HEK293 cell line.
Apoptosis Analysis
Cell Line:
MCF-7 cells
Concentration:
14 μM
Incubation Time:
24 hours
Result:
Induced 35.6% and 2.5% of early and late apoptosis.
体内研究
5β-Dihydrocortisol (0.1-1.0%; 18 d) potentiates the action of topically applied Dexamethasone (0.06%) in raising the intraocular pressure (IOP) in young rabbits.
分子式
C21H32O5
分子量
364.48
CAS号
1482-50-4
运输条件
Room temperature in continental US; may vary elsewhere.