Hesperidin (Synonyms:橙皮苷; Hesperetin 7-rutinoside)
目录号 : KM8020 CAS No. : 520-26-3 纯度 : 98%

Hesperidin (Hesperetin 7-rutinoside) 是一种广泛存在于柑橘类水果中黄烷酮糖苷。Hesperidin 具有多种生物学特性,例如减少炎症介质并发挥重要的抗氧化作用。Hesperidin 还表现出抗肿瘤和抗过敏活性。

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10mg
In-stock
50mg
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100mg
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200mg
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500mg
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1g
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5g 询价 In-stock
10g 询价 In-stock

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生物活性

Hesperidin (Hesperetin 7-rutinoside), a flavanone glycoside, is isolated from citrus fruits. Hesperidin has numerous biological properties, such as decreasing inflammatory mediators and exerting significant antioxidant effects. Hesperidin also exhibits antitumor and antiallergic activities.

体外研究

Hesperidin (5-200 µM; 24-72 h) induces potent cytotoxic effects in human osteosarcoma MG-63 cells.
Hesperidin (5-150 µM; 48 h) induces early and late apoptosis in MG-63 cells.
Hesperidin (10-30 µM) inhibits the activity of COX-2 and iNOS in a dose dependent manner in RAW 264.7 cells activated with LPS.
Hesperidin (0.1 μg/mL; 2 h) decreases the formation of MDA and intracellular ROS, including chondrocyte apoptosis.

Cell Viability Assay

Cell Line: MG-63 cells
Concentration: 0, 5, 25, 50, 100, 150, 200 µM
Incubation Time: 24, 48, 72 hours
Result: Led to time-dependent and concentration-dependent cytotoxic effects, with IC50s of 94.3, 78.6 and 63.3 µM at 24, 48 and 72 h, respectively.

Apoptosis Analysis

Cell Line: MG-63 cells
Concentration: 0, 5, 50, 150 µM
Incubation Time: 48 hours
Result: Increased the percentage of apoptotic cells from 4.7% to 17.9, 34.6 and 68.3% at the concentration of 0, 5, 50 and 150 µM, respectively.
体内研究

Hesperidin (5-80 mg/kg; 2 weeks) significantly suppresses MG-63 tumor growth in mice.
Hesperidin (200 mg/kg; once daily for 28 d) markedly attenuates cartilage destruction and reduces IL-1β and TNF-α levels in a surgically-induced osteoarthritis (OA) rats.

分子式
C28H34O15
分子量
610.56
CAS号
520-26-3
中文名称
橙皮苷;橙皮甙
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : ≥ 33 mg/mL (54.05 mM)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6378 mL 8.1892 mL 16.3784 mL
5 mM 0.3276 mL 1.6378 mL 3.2757 mL
10 mM 0.1638 mL 0.8189 mL 1.6378 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.09 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.09 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

临床试验
科研文献
The molarity calculator equation
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
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