RepSox - CAS:446859-33-2

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RepSox (Synonyms:E-616452; SJN 2511)

目录号 : KM7551 CAS No. : 446859-33-2 纯度 : 98%

Data Sheet 产品使用指南

RepSox (E-616452) 是一种有效，选择性的 TGFβR-1/ALK5 抑制剂，抑制ALK5自磷酸化，IC₅₀ 为 4 nM。

规格	价格	是否有货
5mg		In-stock
10mg		In-stock
50mg		In-stock
100mg		In-stock
200mg	询价	In-stock
500mg	询价	In-stock

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生物活性

RepSox (E-616452) is a potent and selective of the TGFβR-1/ALK5 inhibitor which inhibits ALK5 autophosphorylation with an IC₅₀ of 4 nM.

体外研究

RepSox also inhibits ATP binding to ALK5 with IC₅₀ of 23 nM. RepSox shows potent activity in both binding and cellular assays and exhibits selectivity over p38 mitogen-activated protein kinase. with IC₅₀ of >16 μM. RepSox act s as an inhibitor of the Tgfβ1 kinase. Treatment with 25 μM RepSox almost completely eliminates Smad3 phosphorylation, indicating that RepSox strongly inhibits Tgfβ signaling in somatic cells. RepSox is most effective at replacing Sox2 during days 10-11 after transduction and that therefore cultures of Oct4, Klf4, and cMyc-transduced MEFs give rise to intermediates capable of responding to RepSox treatment. These intermediates appear at day 4 post-transduction and peak at days 10-11. Treatment with RepSox decreased the proportion of cells in G₂/M phase of the cell cycle, indicating it does not increase the proliferation rate of these partially reprogrammed cells.

分子式

C₁₇H₁₃N₅

分子量

287.32

CAS号

446859-33-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 33.33 mg/mL (116.00 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.4804 mL	17.4022 mL	34.8044 mL
5 mM	0.6961 mL	3.4804 mL	6.9609 mL
10 mM	0.3480 mL	1.7402 mL	3.4804 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 7.5 mg/mL (26.10 mM); Clear solution

此方案可获得 ≥ 7.5 mg/mL (26.10 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 75.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 7.5 mg/mL (26.10 mM); Clear solution

此方案可获得 ≥ 7.5 mg/mL (26.10 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 75.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
3.

请依序添加每种溶剂： 5% DMSO 40% PEG300 5% Tween-80 50% saline
Solubility: ≥ 1.67 mg/mL (5.81 mM); Clear solution
4.

请依序添加每种溶剂： 5% DMSO 95% (20% SBE-β-CD in saline)
Solubility: ≥ 1.67 mg/mL (5.81 mM); Clear solution
5.

请依序添加每种溶剂： 1% DMSO 99% saline
Solubility: ≥ 0.33 mg/mL (1.15 mM); Clear solution

科研文献

纯度： 98%

Data Sheet

产品使用指南

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Other Forms of Rapamycin:

KKL 顾客使用本产品发表的 9 篇科研文献

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