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Pseudothymidine is a C-nucleoside analog of thymidine.

Pseudothymidine is a C-nucleoside analog of thymidine. The calculated ΔΔG°₅₀/mod is -0.5 kcal/mol, with a ΔT_m/mod of 0.82°C. For the duplexes containing nine dA-T/ψT pairs, the ΔT_m/mod is -0.9°C and a ΔΔG°₅₀/mod is +1.1 kcal/mol. The modification of the duplex containing 12 consecutive dA-T/ψT base pairs produces a ΔT_m/mod of -0.9°C and a ΔΔG°₅₀/mod of +1.2 kcal/mol.

Room temperature in continental US; may vary elsewhere.

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