TMPD dihydrochloride - CAS:637-01-4

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TMPD dihydrochloride (Synonyms:N,N,N′,N′-四甲基对苯二胺盐酸盐；四甲基对苯二胺二盐酸盐)

目录号 : KM18805 CAS No. : 637-01-4 纯度 : 98%

Data Sheet 产品使用指南

TMPD dihydrochloride，一种易于氧化的化合物，是一种酶促转化的氧化还原活性底物分子。TMPD dihydrochloride 也是一种电子供体，并作为血红素过氧化物酶的还原共底物。TMPD dihydrochloride 也是一种 complex IV 的底物。

规格	价格	是否有货
100mg		In-stock
200mg	询价	In-stock
500mg	询价	In-stock

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生物活性

TMPD dihydrochloride, a readily oxidizable compound, is an enzymatically convert redox active substrate molecule. TMPD dihydrochloride is also an electron donor and serves as a reducing cosubstrate for heme peroxidases. TMPD dihydrochloride is also a complex IV substrate.

体外研究

TMPD (N,N,N′,N′-tetramethyl-para-phenylene-diamine) is used in cell culture and microbiology to differentiate organisms that exhibit cytochrome c oxidase activity and to distinguish between Gram-negative and Gram-positive pathogenic and non-pathogenic bacteria. The concept of TMPD oxidation by microorganisms is widely used and is known as a successful colorimetric indicator for bacterial oxidases, as the radical cation TMPD+˙, formed by oxidation, shows a characteristic deep blue colour. The electrochemical recognition of the TMPD oxidation by bacterial oxidases can be applied to a variety of pathogens.

分子式

C₁₀H₁₈Cl₂N₂

分子量

237.17

CAS号

637-01-4

中文名称

N,N,N′,N′-四甲基对苯二胺盐酸盐；四甲基对苯二胺二盐酸盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:

H₂O : 100 mg/mL (421.64 mM; Need ultrasonic)

DMSO : 16.67 mg/mL (70.29 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.2164 mL	21.0819 mL	42.1638 mL
5 mM	0.8433 mL	4.2164 mL	8.4328 mL
10 mM	0.4216 mL	2.1082 mL	4.2164 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 1.67 mg/mL (7.04 mM); Clear solution

此方案可获得 ≥ 1.67 mg/mL (7.04 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。