Tetraethylammonium chloride - CAS:56-34-8

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Tetraethylammonium chloride (Synonyms:四乙基氯化铵)

目录号 : KM18181 CAS No. : 56-34-8 纯度 : ≥98%

产品说明书产品使用指南

Tetraethylammonium chloride 是一种非选择性的钾通道 (potassium channel) 阻滞剂。Tetraethylammonium chloride 是有机阳离子转运蛋白的良好底物，并具有抗肿瘤特性。

规格	价格	是否有货
500mg		In-stock
1g	询价	In-stock
5g	询价	In-stock

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Other Forms of Rapamycin:

Dendrotoxin K In-stock
QO-40 In-stock
Tetraethylammonium bromide In-stock
VU0134992 In-stock
(+)-KCC2 blocker 1 In-stock

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生物活性

Tetraethylammonium chloride is a non-selective potassium channel blocker. Tetraethylammonium chloride is a good substrate for organic cation transporter (OCTN1). Tetraethylammonium chloride antitumor properties.

体外研究

Tetraethylammonium (0.2-60 mM; 24-72 hours; C6 and 9L glioma cells) treatment inhibits the proliferation of C6 and 9L cells in a dose- and time-dependent manner.
Tetraethylammonium (40 mM; 24-72 hours; C6 and 9L glioma cells) treatment significantly increases apoptosis in cells.
Tetraethylammonium (40 mM; 12-48 hours; C6 and 9L glioma cells) treatment significantly elevates Bax/Bcl-2 protein ratio in a time-dependent manner.
The generation of intracellular ROS increased in C6 and 9L cells by the addition of 20 and 40 mM Tetraethylammonium.

Cell Proliferation Assay

Cell Line:	Rat C6 and 9L glioma cells
Concentration:	0.2 mM, 2 mM, 20 mM, 40 mM and 60 mM
Incubation Time:	24 hours, 48 hours and 72 hours
Result:	Inhibited the proliferation of C6 and 9L cells in a dose- and time-dependent manner.

Apoptosis Analysis

Cell Line:	Rat C6 and 9L glioma cells
Concentration:	40 mM
Incubation Time:	24 hours, 48 hours and 72 hours
Result:	Significantly increased apoptosis in cells.

Western Blot Analysis

Cell Line:	Rat C6 and 9L glioma cells
Concentration:	40 mM
Incubation Time:	12 hours, 24 hours, 48 hours
Result:	The expression of Bax was markedly increased, while that of Bcl-2 showed a decreasing trend 12, 24 and 48 h.

体内研究

Tetraethylammonium (1 mM, 3 mM, and 5 mM) significantly increases the amplitude and frequency of contractility of colon and rectum from rats in longitudinal and circular direction. Tetraethylammonium at 5 mM and 15 mM concentrations shows no effect on histology of colon and rectum from rats that are administered locally with Tetraethylammonium into colon lumen from anus for 10 days.

分子式

C₈H₂₀ClN

分子量

165.70

CAS号

56-34-8

中文名称

四乙基氯化铵

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

DMSO : 100 mg/mL (603.50 mM; Need ultrasonic)

H₂O : ≥ 100 mg/mL (603.50 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	6.0350 mL	30.1750 mL	60.3500 mL
5 mM	1.2070 mL	6.0350 mL	12.0700 mL
10 mM	0.6035 mL	3.0175 mL	6.0350 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (15.09 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (15.09 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (15.09 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (15.09 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (15.09 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (15.09 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。