体外研究 |
SC-43 (0-10 μM; 24-72 hours; HuCCT-1, KKU-100, and CGCCA cells) treatment reveals the anti-proliferative effects in cholangiocarcinoma (CCA) cell lines in a dose-dependent manner after treating 24, 48 and 72 hours respectively.
SC-43 (0-10 μM; 24 hours; HuCCT-1, KKU-100, and CGCCA cells) treatment shows increased sub-G1 cells and G2-M arrest, indicating SC-43 induced differential apoptotic effects in these cell lines.
SC-43 (0-10 μM; 24 hours; HuCCT-1, KKU-100, and CGCCA cells) treatment demonstrates significant increase in cleaved caspase-3 and PARP level.
SC-43 activates SH2 domain-containing phosphatase 1 (SHP-1) activity, leading to p-STAT3 and downstream cyclin B1 and Cdc2 downregulation. SC-43 augments SHP-1 activity by direct binding to N-SH2 and relief of its autoinhibition.
Cell Viability Assay
Cell Line: |
HuCCT-1, KKU-100, and CGCCA cells |
Concentration: |
0 μM, 0.25 μM, 0.5 μM, 0.75 μM, 1 μM, 2.5 μM, 5 μM, 10 μM |
Incubation Time: |
24 hours, 48 hours, 72 hours |
Result: |
Revealed the anti-proliferative effects in CCA cell lines in a dose-dependent manner after treating 24, 48 and 72 hours respectively.
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Cell Cycle Analysis
Cell Line: |
HuCCT-1, KKU-100, and CGCCA cells |
Concentration: |
0 μM, 1 μM, 2.5 μM, 5 μM, 10 μM |
Incubation Time: |
24 hours |
Result: |
Showed increased sub-G1 cells and G2-M arrest.
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Western Blot Analysis
Cell Line: |
HuCCT-1, KKU-100, and CGCCA cells |
Concentration: |
0 μM, 1 μM, 2.5 μM, 5 μM, 10 μM |
Incubation Time: |
24 hours |
Result: |
Demonstrated significant increase in cleaved caspase-3 and PARP level.
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