Guanidine hydrochloride (Synonyms:盐酸胍; Guanidinium chloride; Aminoformamidine hydrochloride)
目录号 : KM19973 CAS No. : 50-01-1 纯度 : ≥98%

Guanidine hydrochloride (Guanidinium chloride) 是一种强离液剂,也是一种蛋白质的强变性剂。

规格 价格 是否有货 数量
500mg
In-stock
10g
In-stock
50g
In-stock

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生物活性

Guanidine hydrochloride (Guanidinium chloride) a strong chaotrope, is also a strong denaturant of proteins.

体外研究

Guanidine hydrochloride at low concentrations refolds acid-unfolded apomyoglobin and cytochrome c, stabilizing the molten globule state, i.e. a compact denatured state with a significant amount of secondary structure, but substantially disordered tertiary structure. Guanidine hydrochloride (> 1 M) causes co-operative unfolding of the molten globule state.
A considerable degree of ordered structure remains due to the presence of disulfide bonds, which Guanidine hydrochloride does not affect.
Guanidine hydrochloride at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI] element from yeast cells. 5 mM Guanidine hydrochloride in growth media cures [PSI] and other prions of yeast. 5 mM Guanidine hydrochloride significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine hydrochloride also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase.

体内研究

A significant reduction in the death rate of infant mice infected with ten LD50) of coxsackievirus A16 is observed when they are treated 58 h after infection with two injections of Guanidine hydrochloride at 145 mg/kg per injection.

分子式
CH6ClN3
分子量
95.53
CAS号
50-01-1
中文名称
盐酸胍
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

2-8˚C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 100 mg/mL (1046.79 mM; Need ultrasonic)

DMSO : ≥ 100 mg/mL (1046.79 mM)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 10.4679 mL 52.3396 mL 104.6792 mL
5 mM 2.0936 mL 10.4679 mL 20.9358 mL
10 mM 1.0468 mL 5.2340 mL 10.4679 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (1046.79 mM); Clear solution; Need ultrasonic

     

  • 2.

    请依序添加每种溶剂: 10% DMSO → 40% PEG300 → 5% Tween-80 → 45% saline

    Solubility: ≥ 2.08 mg/mL (21.77 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (21.77 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

     
  • 3.

    请依序添加每种溶剂: 10% DMSO → 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (21.77 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (21.77 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

     
  • 4.

    请依序添加每种溶剂: 10% DMSO → 90% corn oil

    Solubility: ≥ 2.08 mg/mL (21.77 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (21.77 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

临床试验
科研文献
The molarity calculator equation
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
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