Tetrahydrofolic acid - CAS:135-16-0

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Tetrahydrofolic acid (Synonyms:四氢叶酸; L-5,6,7,8-Tetrahydrofolic acid; L-Tetrahydrofolic acid)

目录号 : KM8956 CAS No. : 135-16-0 纯度 : 96%

Data Sheet 产品使用指南

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) 是一种具有生物活性的维生素 B9 叶酸衍生物，是氨基酸，核酸和脂质的一个碳原子的供体。Tetrahydrofolic acid 用作游离甲醛的受体，产生 5,10-亚甲基四氢叶酸-四氢叶酸。

规格	价格	是否有货
10mg		In-stock
25mg		In-stock
50mg	询价	In-stock
100mg	询价	In-stock

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生物活性

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid.

体外研究

Tetrahydrofolic acid (0-200 µM; 3 days; Adh5 DT40 cells) exposure is cytotoxic to Adh5- and Fanconi anemia (FA)-deficient cells due to the accumulation of extensive DNA damage and chromosome breaks.
Tetrahydrofolic acid (0-100 µM; 16 hours; Adh5 DT40 cells) treatment strongly promots FANCD2 and ser139-H2AX focus formation in Adh5 cells in a dose-dependent manner.
Tetrahydrofolic acid exposure activates the DNA damage response (DDR) due to uncontrolled activity of the thymidylate synthase enzyme, which causes a depletion of essential nucleotides, and promotes repair by a homologous recombination mechanism.

Cell Viability Assay

Cell Line:	Adh5 DT40 cells
Concentration:	0-200 µM
Incubation Time:	3 days
Result:	Viability of Adh5 DT40 cells rapidly dropped.

Western Blot Analysis

Cell Line:	Adh5 DT40 cells
Concentration:	0-200 µM
Incubation Time:	16 hours
Result:	Strongly promoted FANCD2 and ser139-H2AX focus formation in Adh5 cells in a dose-dependent manner.

体内研究

Tetrahydrofolic acid (62.5 mg/kg; intraperitoneal injection; daily; Adh5 mice) treatment perturbs the hematopoiesis of hematopoietic cells, increases ser139-H2AX phosphorylation, and decreases the survival of progenitor cells (HSPCs) suggesting that excess Tetrahydrofolic acid could be mutagenic and genotoxic to bone marrow cells.

Animal Model:	Adh5 mice
Dosage:	62.5 mg/kg
Administration:	Intraperitoneal injection; daily
Result:	Perturbed hematopoiesis, increased ser139-H2AX phosphorylation, and decreased the survival of progenitor cells (HSPCs).

分子式

C₁₉H₂₃N₇O₆

分子量

445.43

CAS号

135-16-0

中文名称

四氢叶酸

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light, stored under nitrogen

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

溶解性数据

In Vitro:

DMSO : 20.83 mg/mL (46.76 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.2450 mL	11.2251 mL	22.4502 mL
5 mM	0.4490 mL	2.2450 mL	4.4900 mL
10 mM	0.2245 mL	1.1225 mL	2.2450 mL

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (4.67 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (4.67 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。