Coenzyme FO - CAS:37333-48-5

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Coenzyme FO

目录号 : KM7277 CAS No. : 37333-48-5

Data Sheet 产品使用指南

Coenzyme FO 是一种脱氮黄素发色团，在产甲烷途径中起着重要的氢化物受体/供体作用。

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生物活性

Coenzyme FO, a deazaflavin chromophore, acts as an important hydride acceptor/donor in the central methanogenic pathway.

体外研究

The formation of Coenzyme FO is mediated by two separate radical SAM active sites, one each in the CofG and CofH enzymes or both in the FbiC enzyme. These two radical SAM domains constitute the functional domains of Fo synthase. F420 is biosynthesized in Methanocaldococcus jannaschii by the action of eight enzymes with the formation of the deazaflavin chromophore (Fo) as the remaining unsolved step.
Coenzyme F420 is the central low-redox-potential electron carrier in methanogenic metabolism. Coenzyme F420 is reduced under hydrogen by the action of F420-dependent hydrogenase.
Coenzyme F420 acts as a hydride transfer coenzyme for an F420-specific glucose-6-phosphate dehydrogenase (Fgd) in mycobacteria. Coenzyme F420 is found in all methanogenic and certain nonmethanogenic archaea, where it participates in energy metabolism, NADP reduction, oxygen detoxification, and sulfite reduction. By converting NO2 back to NO with F420H2, M. tuberculosis could decrease the effectiveness of antibacterial action of macrophages.

分子式

C₁₆H₁₇N₃O₇

分子量

363.32

CAS号

37333-48-5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 5 mg/mL (13.76 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.7524 mL	13.7620 mL	27.5239 mL
5 mM	0.5505 mL	2.7524 mL	5.5048 mL
10 mM	0.2752 mL	1.3762 mL	2.7524 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 1 mg/mL (2.75 mM); Suspended solution; Need ultrasonic

此方案可获得 1 mg/mL (2.75 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 10.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 0.53 mg/mL (1.46 mM); Suspended solution; Need ultrasonic

此方案可获得 0.53 mg/mL (1.46 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 5.3 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。