NSC668394 - CAS:382605-72-3

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NSC668394

目录号 : KM5488 CAS No. : 382605-72-3

Data Sheet 产品使用指南

NSC668394 是一种有效的 ezrin (Thr567) 磷酸化抑制剂，K_d 值为 12.59 μM。NSC668394 主要通过与 ezrin 结合来抑制 PKCΙ 导致的 ezrin T567 磷酸化。NSC668394 可用于预防肿瘤转移。

规格	价格	是否有货	数量
5mg		In-stock
10mg		In-stock
25mg		In-stock
50mg		In-stock
100mg		In-stock

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生物活性

NSC668394 is a potent ezrin (Thr567) phosphorylation inhibitor, with a K_d of 12.59 μM. NSC668394 inhibit ezrin T567 phosphorylation caused by PKCΙ primarily via their binding to ezrin. NSC668394 can be used to prevent tumor metastasis.

体外研究

NSC668394 (10 μM; pretreated for 15 min) inhibits ezrin T567 phosphorylation (IC₅₀=8.1 μM) and actin binding in vitro.
NSC668394 (1-10 μM; 2-6 h) inhibits ezrin-mediated invasion by K7M2 osteosarcoma (OS) cells on the HUVEC monolayer.
NSC668394 (20 μM) causes significant decrease in growth in JM1 and JM2 rat hepatoma cell lines.
NSC668394 (10 μM) reduces cell motility phenotypes in zebrafish.

Western Blot Analysis

Cell Line:	K7M2 OS cells
Concentration:	10 μM
Incubation Time:	6 hours
Result:	Inhibited T567 phosphorylation and actin binding of endogenous ezrin without altering cellular ezrin levels.

体内研究

NSC668394 (0.226 mg/kg/day; i.p. 5-days a week) inhibits ezrin-dependent in vivo OS metastatic growth in mouse lung.

Animal Model:	Female BALB/c and SCID/Beige mice were injected with K7M2 or MNNG-HOS tumor cells
Dosage:	0.226 mg/kg/day
Administration:	I.p. 5-days a week for 66 days
Result:	Showed an increase in survival. Decreased the number of the green fluorescent protein (GFP)-expressing metastatic foci in the lung tissues.

分子式

C₁₇H₁₂N₂O₃Br₂

分子量

452.10

CAS号

382605-72-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 3.85 mg/mL (8.52 mM; ultrasonic and warming and heat to 80°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.2119 mL	11.0595 mL	22.1190 mL
5 mM	0.4424 mL	2.2119 mL	4.4238 mL
10 mM	---	---	---

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

科研文献

•Research Square Preprint. 2021 Oct.

Data Sheet

产品使用指南

摩尔计算器
稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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分子量*

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

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浓度

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Other Forms of Rapamycin:

KKL 顾客使用本产品发表的 1 篇科研文献

The molarity calculator equation

The dilution calculator equation