Cl-amidine is a bioavailable haloacetamidine-based compound that inhibits all the active PAD isozymes with near equal potency (kinact/KI=13,000 M-1•min-1 for PAD4).
Cl-amidine (0, 5, 10, 15, 20, 25, 50 μg/mL, 24 hours) induces apoptosis in TK6 lymphoblastoid cells and HT29 colon cancer cells in a dose-dependent manner. Interestingly, the colon cancer cell line (HT29) is relatively resistant to apoptosis caused by Cl-amidine.
Cl-Amidine prevents histone 3 citrullination and neutrophil extracellular trap formation, and improves survival in a murine sepsis model.
Apoptosis Analysis.
Cell Line: |
TK6 lymphoblastoid cells and HT29 colon cancer cells. |
Concentration: |
0, 5, 10, 15, 20, 25, 50 μg/mL. |
Incubation Time: |
24 h. |
Result: |
Induced apoptosis dose-dependently. |