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2-Hydroxyestradiol, a metabolite of 17β-estradiol with minimal estrogenic activity, possesses antioxidant effects and reacts with DNA to form stable adducts and exerts genotoxicity.

2-Hydroxyestradiol induces phenotypical changes indicative of neoplastic transformation.
2-Hydroxyestradiol (2-OHE₂) is capable of in vitro transforming a HBEC, MCF-10F cell line, that is estrogen receptor-a (ERα) negative and ERβ positive.
2-Hydroxyestradiol induces oxidative DNA damage and apoptosis in human mammary epithelial cells.

2-Hydroxyestradiol attenuated the development of obesity and improved endothelial function, decreased nephropathy, decreased the severity of diabetes, lowered arterial blood pressure, and reduced plasma cholesterol.

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