βeta-Zearalenol (0-200 μM; 24 hours) inhibits BGC proliferation in a dose-dependent manner, with an IC50 of 25 μM.
βeta-Zearalenol (0-100 μM; 72 hours) shows high toxic with an IC50 of 15.2 μM in the HepG2 cells, synergistic effect of β-ZOL+ZEN or β-ZOL+α-ZOL with IC50 values of 19.8 uM, 16.8 uM, respectively.
βeta-Zearalenol (0-10 μM; 72 hours) has the highest inhibition effect on the all three investigated cytokines: IL-8, IL-1β and TNF-α by comparation with ZEA or α-Zearalenol.
Cell Viability Assay
Cell Line: |
HepG2 cells |
Concentration: |
0-100 μM |
Incubation Time: |
72 hours |
Result: |
Shown a dose-dependent reduction of viability of cells treated with Beta-Zearalanol. |
Cell Proliferation Assay
Cell Line: |
BGC cells |
Concentration: |
0 μM, 5 μM, 10 μM, 15 μM, 25 μM, 50 μM, 100 μM, and 200 μM |
Incubation Time: |
24 hours |
Result: |
Inhibited cell proliferation in a dose-dependent manner. |
RT-PCR
Cell Line: |
HepG2 cells |
Concentration: |
1 μM, 5 μM, 10 μM |
Incubation Time: |
72 hours |
Result: |
Had the higher cytotoxic effect in comparation with α-Zearalenol. |