Camphor induces fibroblast proliferation through the PI3K/AKT and ERK signaling pathways.
The MTT assay results show that 32.5, 65, 130, and 260 μM Camphor increase fibroblast viability to 108.9±6.6%, 118.6±2.8%, 127.7±4.2%, and 131.6±7.2%, respectively, compared to 0 μM Camphor treatment.
Camphor (0-260 μM) treatment for 24 hours increases the generation of ROS by up to 17.97% compared to 5.04% in the no-treatment control.
Camphor (0-260 μM, 24 hours) induces the phosphorylation of PI3K, AKT, ERK, and 4EBP1 in a dose- and time-dependent manner.
Cell Viability Assay
Cell Line: |
Primary dermal fibroblast cells |
Concentration: |
0-260 μM |
Incubation Time: |
24 hours |
Result: |
32.5, 65, 130, and 260 μM increased fibroblast viability to 108.9±6.6%, 118.6±2.8%, 127.7±4.2%, and 131.6±7.2%, respectively, compared to 0 μM treatment. |
Western Blot Analysis
Cell Line: |
Primary dermal fibroblast cells |
Concentration: |
0-260 μM |
Incubation Time: |
24 hours |
Result: |
Induced the phosphorylation of PI3K, AKT, ERK, and 4EBP1, a repressor of mRNA translation and mTOR substrate, in a dose- and time-dependent manner.
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