TAT-DEF-Elk-1 TFA - KKL Med Inc.

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TAT-DEF-Elk-1 TFA

目录号 : KM15167

Data Sheet 产品使用指南

TAT-DEF-Elk-1 TFA (TDE TFA) 是 Elk-1 的一种可穿透细胞的肽抑制剂，通过模仿和特异性干扰 Elk-1 的 DEF 结构域来实现抑制。TAT-DEF-Elk-1 TFA 阻止 Elk-1 磷酸化并防止 Elk-1 核易位，且不会干扰 ERK 或 MSK1 活化。TAT-DEF-Elk-1 TFA 是分析神经元可塑性发展过程中 Elk-1 在此过程中的作用的有用工具。

规格	价格	是否有货	数量
1mg		In-stock
5mg		In-stock
10mg		In-stock

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生物活性

TAT-DEF-Elk-1 TFA (TDE TFA) is a cell-penetrating peptide inhibitor of Elk-1, mimics and specifically interferes with the DEF domain of Elk-1. TAT-DEF-Elk-1 TFA blocks Elk-1 phosphorylation and prevents Elk-1 nuclear translocation without interfering with ERK nor MSK1 activation. TAT-DEF-Elk-1 TFA is a useful tool to analyze the role of Elk-1 in this process during the development of neuronal plasticity.

体外研究

Elk-1 phosphorylation on Ser383/389 has a dual function and triggers both Elk-1 nuclear translocation and SRE-dependent gene expression.
TAT-DEF-Elk-1 TFA (5 μM; 1 hour) specifically inhibits glutamate-induced elk-1 activation and does not interfer with ERK, MSK-1, or CREB phosphorylation.
TAT-DEF-Elk-1 TFA (5-10 μM; 2 hour) treatment shows a significant inhibition of c-Fos, Zif268 and JunB, but has no effects on c-Jun expression.

Western Blot Analysis

Cell Line:	Neurons
Concentration:	5 μM; 10 μM
Incubation Time:	1 hour
Result:	Decreased elk-1 expression and had no effects on ERK, MSK-1, or CREB phosphorylation.

RT-PCR

Cell Line:	Primary striatal neurons
Concentration:	5 μM
Incubation Time:	2 hour
Result:	Decreased c-Fos, Zif268 and JunB mRNA level but did not effect c-Jun.

体内研究

TAT-DEF-Elk-1 TFA (intraperitoneal injection; 1mg/kg; daily; 14 days) reflects antidepressant efficacy in mice, it decreases immobility similar to the reference antidepressants fluoxetine and desipramine (DMI).

Animal Model:	C57Bl6 mice (3-6 months old males) are subjected to social defeat stress
Dosage:	1 mg/kg
Administration:	Intraperitoneal injection; daily; 14 days
Result:	Reversed social-defeat induced decrease of hippocampal Bdnf expression by repeated TDE administration.