3,4-Dihydroxybenzylamine hydrobromide - CAS:16290-26-9

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3,4-Dihydroxybenzylamine hydrobromide (Synonyms:NSC 263475 hydrobromide)

目录号 : KM14027 CAS No. : 16290-26-9 纯度 : ≥95%

Data Sheet 产品使用指南

3,4-Dihydroxybenzylamine hydrobromide (NSC 263475 hydrobromide) 是一种结构改良的多巴胺类似物细胞毒物，抑制黑色素瘤细胞中 DNA 聚合酶 (DNA polymerase) 活性。3,4-Dihydroxybenzylamine hydrobromide 在不同程度的酪氨酸酶活性的黑色素瘤细胞系中显示不同的生长抑制活性。

规格	价格	是否有货
100mg		In-stock
200mg	询价	In-stock
500mg	询价	In-stock

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生物活性

3,4-Dihydroxybenzylamine hydrobromide (NSC 263475 hydrobromide) is an improved dopamine analog cytotoxic and inhibits DNA polymerase activity in melanoma cells. 3,4-Dihydroxybenzylamine hydrobromide (NSC 263475 hydrobromide) displays growth inhibitory activity in melanoma cell lines with varying degrees of tyrosinase activity.

体外研究

3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) inhibits the growth of all melanoma cell lines with an IC₅₀ of 100 μM, while the SK-MELB cell line exhibits an IC₅₀ of 122 μM. The IC₅₀ values are 30, 84,90, and 68 μM for Human SK-MEL-30, SK-MEL-2, SK-MEL-3,RPMI-7951 cells, respectively. It exhibits IC₅₀values of 10, 25, 67, and 184 μM for S91A,S91B, L1210, and SCC-25 cells, respectively.
3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) cytotoxicity in SK-MEL-2 and SK-MEL-28 cell lines can be enhanced by application of buthionine sulfoximine (BSO). BSO treatment sensitizes marginally pigmented cells to the effects of 3,4-DHBA, and the presence of decreses IC₅₀ values of 3,4-DHBA by 127% in the SK-MEL-28 cell line and by 148% in the SK-MEL-2 cell line.

Cell Viability Assay

Cell Line:	Human and Murine Melanoma Cell Lines; Non-Melanoma Cell Lines
Concentration:	1 μM-10 mM
Incubation Time:	48 hours
Result:	Inhibited melanoma cell Lines growth.

体内研究

3,4-Dihydroxybenzylamine hydrobromide (intraperitoneal injection; 1000 mg/kg; 7 days) has the least toxic effect in non-tumor-bearing B6D2F1 mice,and mice is tolerated at this dose.
3,4-Dihydroxybenzylamine hydrobromide (intraperitoneal injection; 200-800 mg/kg; 21 days) has different effects at different doses, the median life-span are 17, 24.5, 26, 29 and 25 days for 0 mg/kg, 200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg, respectively.

Animal Model:	C57BL/6 mice with B6D2F1 cells
Dosage:	200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg
Administration:	Intraperitoneal injection
Result:	Exhibited increased life-span of 44%,46%, 70% and 50% for 200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg, respectively.

分子式

C₇H₁₀BrNO₂

分子量

220.06

CAS号

16290-26-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 83.33 mg/mL (378.67 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.5442 mL	22.7211 mL	45.4422 mL
5 mM	0.9088 mL	4.5442 mL	9.0884 mL
10 mM	0.4544 mL	2.2721 mL	4.5442 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (9.45 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (9.45 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (9.45 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (9.45 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (9.45 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (9.45 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。