Benserazide hydrochloride - CAS:14919-77-8

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Benserazide hydrochloride (Synonyms:盐酸苄丝肼; Serazide; Ro 4-4602)

目录号 : KM13556 CAS No. : 14919-77-8 纯度 : 98%

Data Sheet 产品使用指南

Benserazide hydrochloride (Serazide) 常用于帕金森病，是一种芳香族 L-氨基酸脱羧酶（AADC）的抑制剂。

规格	价格	是否有货
500mg		In-stock
1g		In-stock
5g		In-stock
10g	询价	In-stock
50g	询价	In-stock

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生物活性

Benserazide hydrochloride (Serazide) is commonly used in Parkinson's disease and is an inhibitor of peripheral aromatic L-amino acid decarboxylase (AADC).

体外研究

Benserazide hydrochloride (BH) and Levodopa (LD) individually and in combination (Benserazide hydrochloride + LD) (25 μM; 0 hour, 12 hours, 24 hours and 168 hours; SH-SY5Y) treatment inhibit protein aggregation and have the ability to minimise the amyloid-induced cytotoxicity in human neuroblastoma cell line. Benserazide hydrochloride and LD both can act as efficient inhibitors of the formation of cytotoxic HSA aggregates, and the inhibitory effects are more pronounced when both of these drugs are added simultaneously.

Cell Viability Assay

Cell Line:	SH-SY5Y cells
Concentration:	25 μM
Incubation Time:	0 hour, 12 hours, 24 hours and 168 hours
Result:	Enhanced cell viability, and inhibited the formation of cytotoxic human serum albumin (HSA) aggregates.

体内研究

Benserazide (5-50 mg/kg; intraperitoneal injection; male Wistar rats) treatment of 6-OHDA-lesioned rats increases exogenous L-DOPA-derived extracellular DA levels, the time to reach the peak DA levels are significantly prolong by Benserazide dose-dependently. The AADC activity in the denervates striatal tissues shows a significant decrease by 10 mg/kg and 50 mg/kg Benserazide. Benserazide reduces the central AADC activity in the striatum of rats with nigrostriatal denervation, which leads to changes in the metabolism of exogenous L-DOPA.

Animal Model:	Male Wistar rats with 6-hydroxydopamine (6-OHDA) (8 Ag/4 Al)
Dosage:	5 mg/kg, 10 mg/kg or 50 mg/kg (Pharmacokinetic study)
Administration:	Intraperitoneal injection
Result:	Increased in exogenous L-DOPA-derived extracellular DA levels, the time to reach the peak DA levels were significantly prolonged in a dose-dependent fashion. The AADC activity in the denervated striatal tissues showed a significant decreased by 10 mg/kg and 50 mg/kg.

分子式

C₁₀H₁₆ClN₃O₅

分子量

293.70

CAS号

14919-77-8

中文名称

盐酸苄丝肼；三羟基丝氨酰肼；色拉肼；丝氯酰肼；羟苄丝肼

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 100 mg/mL (340.48 mM; Need ultrasonic)

H₂O : ≥ 50 mg/mL (170.24 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.4048 mL	17.0242 mL	34.0483 mL
5 mM	0.6810 mL	3.4048 mL	6.8097 mL
10 mM	0.3405 mL	1.7024 mL	3.4048 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (340.48 mM); Clear solution; Need ultrasonic
2.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (7.08 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.08 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
3.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (7.08 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.08 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
4.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (7.08 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.08 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。