Crotonoside - CAS:1818-71-9

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Crotonoside (Synonyms:巴豆苷; Isoguanosine)

目录号 : KM13054 CAS No. : 1818-71-9 纯度 : 98%

Data Sheet 产品使用指南

Crotonoside is isolated from Chinese medicinal herb, Croton. Crotonoside inhibits FLT3 and HDAC3/6, exhibits selective inhibition in acute myeloid leukemia (AML) cells. Crotonoside could be a promising new lead compound for the treatment of AML.

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10mg		In-stock
20mg		In-stock
50mg	询价	In-stock
100mg	询价	In-stock

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生物活性

体外研究

Crotonoside (0-200 μM;) selectively inhibits the viability of AML cell line MV4-11, MOLM-13 (with FLT3-ITD mutant) and KG-1 (without FLT3-ITD mutant) in a dose-dependent manner with an IC₅₀ of 11.6 μM, 12.7 μM and 17.2 μM, respectively.
Crotonoside (0-100μM; 7 hours) inhibits the phosphorylation of FLT3 Erk1/2, Akt/mTOR and STAT5 is strongly inhibited by crotonoside at higher concentration of 12.5 μM in a concentration-dependent manner.
Crotonoside (0-100 μM; 12 hours) exhibits a dose-dependent increase in the percentage of G0/G1 phase and a dose-dependent decrease in the percentage of G2/M and S phases cells.
Crotonoside (0-100 μM; 24 hours) leads to concentration-dependent changes in the number of apoptotic MV4-11 cells, results in a dose-dependent decrease in the level of pro-caspase-3 and a dose-dependent increase in the level of the cleaved caspase-3 fragments.

Cell Viability Assay

Cell Line:	AML cell line MV4-11, MOLM-13 and KG-1 cells
Concentration:	0-200 μM
Incubation Time:	72 hours
Result:	Inhibited AML cells growth than other cell lines tested.

Western Blot Analysis

Cell Line:	MV4-11 cells
Concentration:	0 μM, 12.5 μM, 25 μM, 50 μM
Incubation Time:	7 hours
Result:	Inhibited AML cells growth than other cell lines tested.

Cell Cycle Analysis

Cell Line:	MV4-11 cells
Concentration:	0 μM, 12.5 μM, 25 μM, 50 μM
Incubation Time:	12 hours
Result:	Induced cell cycle arrest in G0/G1.

Apoptosis Analysis

Cell Line:	MV4-11 cells
Concentration:	0 μM, 12.5 μM, 25 μM, 50 μM
Incubation Time:	24 hours
Result:	Induced MV4-11 cell apoptosis.

体内研究

Crotonoside(intraperitoneal and intravenous injection; 70 mg/kg, 35 mg/kg; once daily) induces a significant antitumor activity and inhibited xenograft tumor progress as compared to treatment with vehicle.

Animal Model:	NOD-SCID mice with MV4-11 cells
Dosage:	70 mg/kg, 35 mg/kg
Administration:	Intraperitoneal and intravenous injection; 70 mg/kg, 35 mg/kg; once daily
Result:	Produced significant AML tumor inhibition rates of 93.5% at 70 mg/kg.

分子式

C₁₀H₁₃N₅O₅

分子量

283.24

CAS号

1818-71-9

中文名称

巴豆苷

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 25 mg/mL (88.26 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.5306 mL	17.6529 mL	35.3057 mL
5 mM	0.7061 mL	3.5306 mL	7.0612 mL
10 mM	0.3531 mL	1.7653 mL	3.5306 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (7.34 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.34 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (7.34 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.34 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (7.34 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.34 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。